| Application of a ready-to-use cell sensor for dioxins and dioxin-like compounds screening in foodstuffs | |
Zhang, Songyan; Zhang, Hang; Chen, Yangsheng ; Yang, Haoyi; Qu, Junle; Xu, Li; Zheng, Liping; Xie, Qunhui; Jiang, Yousheng; Zhang, Jianqing; Bi, Wenchuan; Zhang, Jian; Zhao, Bin
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| 2022-09-10 | |
| Source Publication | SCIENCE OF THE TOTAL ENVIRONMENT
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| ISSN | 0048-9697 |
| Volume | 838Issue:0Pages:156227 |
| Abstract | Dioxins and dioxin-like compounds (DLCs) in foodstuffs are closely related to human health. As China is the largest food-consuming country, there is a potentially large demand for screening bioassays that are rapid, cost-effective and capable of determining dioxins and DLCs in foodstuffs. CBG2.8D is a reporter gene-based recombinant cell sensor that was recently developed for determining dioxin and DLCs in ambient and seafood samples. In this study, we established a bioanalytical method with this ready-to-use cell sensor for the bioanalysis of dioxins and DLCs in different types of meat samples. Twenty-nine samples from three typical types of meat (beef, pork and fish) were collected and subjected to both instrumental analysis and a CBG2.8D bioassay. The intra-and inter-lab reproducibility of the bioassay was investigated and the coefficients of variation (CVs) were lower than 25%, suggesting that the cell sensor had a good reproducibility for the meat samples. Based on the correlation equation and coefficient obtained by comparing the data from the instrumental analysis and CBG2.8D bioassay, we found that this method had better performance with pork and fish than with beef. The compliance rate was also determined by comparing the results from the instrumental analysis and there were no false results for the pork and fish samples. Lastly, a complete operation procedure was summarized as a guideline for practical application. In conclusion, the CBG2.8D cell sensor exhibits excellent stability and is capable of screening dioxins and DLCs in meat samples. |
| Department | 环境化学与生态毒理学国家重点实验室 ; 环境化学与生态毒理学国家重点实验室 |
| Keyword | ARYL-HYDROCARBON RECEPTOR DIBENZO-P-DIOXINS DIETARY-INTAKE BIOASSAY PCBS PCDD/FS FOOD BIPHENYLS FISH ACTIVATION |
| Document Type | 期刊论文 |
| Identifier | https://ir.rcees.ac.cn/handle/311016/47467 |
| Collection | 环境化学与生态毒理学国家重点实验室 |
| Affiliation | 1.Shenzhen Univ, Hlth Sci Ctr, Engn Lab Shenzhen Nat Small Mol Innovat Drugs, Shenzhen, Peoples R China 2.Shenzhen Univ, Coll Phys & Optoelect Engn, Shenzhen, Peoples R China 3.Chinese Acad Sci, Res Ctr Ecoenvironm Sci, State Key Lab Environm Chem & Ecotoxicol, Beijing, Peoples R China 4.Shenzhen Ctr Dis Control & Prevent, Shenzhen, Peoples R China 5.Shenzhen Univ, Hlth Sci Ctr, Engn Lab Shenzhen Nat Small Mol Innovat Drugs, Shenzhen 518055, Pe |
| Recommended Citation GB/T 7714 | Zhang, Songyan,Zhang, Hang,Chen, Yangsheng,et al. Application of a ready-to-use cell sensor for dioxins and dioxin-like compounds screening in foodstuffs[J]. SCIENCE OF THE TOTAL ENVIRONMENT,2022,838(0):156227. |
| APA | Zhang, Songyan.,Zhang, Hang.,Chen, Yangsheng.,Yang, Haoyi.,Qu, Junle.,...&Zhao, Bin.(2022).Application of a ready-to-use cell sensor for dioxins and dioxin-like compounds screening in foodstuffs.SCIENCE OF THE TOTAL ENVIRONMENT,838(0),156227. |
| MLA | Zhang, Songyan,et al."Application of a ready-to-use cell sensor for dioxins and dioxin-like compounds screening in foodstuffs".SCIENCE OF THE TOTAL ENVIRONMENT 838.0(2022):156227. |
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