| Constructing an MCF-7 breast cancer cell-based transient transfection assay for screening RAR? (Ant)agonistic activities of emerging phenolic compounds | |
Xu, Hanqing ; Su, Jiahui; Ku, Tingting; Liu, Qian S.; Liang, Jiefeng; Ren, Zhihua; Zhou, Qunfang; Jiang, Guibin
| |
| 2022-08-05 | |
| Source Publication | JOURNAL OF HAZARDOUS MATERIALS
 |
| ISSN | 0304-3894 |
| Volume | 435Issue:0Pages:129024 |
| Abstract | The screening of compounds with endocrine disrupting effects has been attracting increasing attention due to the continuous release of emerging chemicals into the environment. Testing the (ant)agonistic activities of these chemicals on the retinoic acid receptor alpha (RAR alpha), a vital nuclear receptor, is necessary to explain their perturbation in the endocrine system in vivo. In the present study, MCF-7 breast carcinoma cells were transiently transfected with a RAR alpha expression vector (pEF1 alpha-RAR alpha-RFP) and a reporter vector containing a retinoic acid reaction element (pRARE-TA-Luc). Under optimized conditions, the performance of the newly constructed system was evaluated for its feasibility in screening the (ant)agonistic effects of emerging phenolic compounds on RAR alpha. The results showed that this transient transfection cell model responded well to stimulation with (ant) agonists of RAR alpha, and the EC50 and IC50 values were 0.87 nM and 2.67 mu M for AM580 and Ro41-5253, respectively. Its application in testing several emerging phenolic compounds revealed that triclosan (TCS) and tetrabromobisphenol A (TBBPA) exerted notable RAR alpha antagonistic activities. This newly developed bioassay based on MCF-7 is promising in identifying the agonistic or antagonistic activities of xenobiotics on RAR alpha and has good potential for studying RAR alpha signaling-involved toxicological effects of emerging chemicals of concern. |
| Department | 环境化学与生态毒理学国家重点实验室 ; 环境化学与生态毒理学国家重点实验室 |
| Keyword | ACID RECEPTOR-ALPHA RETINOIC ACID GENE-EXPRESSION DEPENDENT TRANSCRIPTION GROWTH-INHIBITION IN-VITRO RXR CONTAMINANTS ANTAGONIST ACTIVATION |
| Document Type | 期刊论文 |
| Identifier | https://ir.rcees.ac.cn/handle/311016/47491 |
| Collection | 环境化学与生态毒理学国家重点实验室 |
| Corresponding Author | Zhou, Qunfang |
| Affiliation | 1.Chinese Acad Sci, Res Ctr Ecoenvironm Sci, State Key Lab Environm Chem & Ecotoxicol, Beijing 100085, Peoples R China 2.Wenzhou Univ, Natl & Local Joint Engn Res Ctr Ecol Treatment Tec, Wenzhou 325035, Peoples R China 3.Univ Chinese Acad Sci, Coll Resources & Environm, Beijing 100049, Peoples R China 4.Shanxi Univ, Coll Environm & Resources, Res Ctr Environm & Hlth, Taiyuan 030006, Peoples R China 5.Univ Chinese Acad Sci, Sino Danish Coll, Beijing 100049, Peoples R China |
| Recommended Citation GB/T 7714 | Xu, Hanqing,Su, Jiahui,Ku, Tingting,et al. Constructing an MCF-7 breast cancer cell-based transient transfection assay for screening RAR? (Ant)agonistic activities of emerging phenolic compounds[J]. JOURNAL OF HAZARDOUS MATERIALS,2022,435(0):129024. |
| APA | Xu, Hanqing.,Su, Jiahui.,Ku, Tingting.,Liu, Qian S..,Liang, Jiefeng.,...&Jiang, Guibin.(2022).Constructing an MCF-7 breast cancer cell-based transient transfection assay for screening RAR? (Ant)agonistic activities of emerging phenolic compounds.JOURNAL OF HAZARDOUS MATERIALS,435(0),129024. |
| MLA | Xu, Hanqing,et al."Constructing an MCF-7 breast cancer cell-based transient transfection assay for screening RAR? (Ant)agonistic activities of emerging phenolic compounds".JOURNAL OF HAZARDOUS MATERIALS 435.0(2022):129024. |
| Files in This Item: | Download All | |||||
| File Name/Size | DocType | Version | Access | License | ||
| Constructing an MCF-（2336KB） | 期刊论文 | 出版稿 | 开放获取 | CC BY-NC-SA | View Download | |
Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.
Edit Comment