| Silver Nanoparticles Induce Apoptosis in HepG2 Cells through Particle-Specific Effects on Mitochondria | |
Wang, Fengbang ; Chen, Zihan; Wang, Yuanyuan; Ma, Chunyan; Bi, Lei; Song, Maoyong; Jiang, Guibin
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| 2022-05-03 | |
| Source Publication | ENVIRONMENTAL SCIENCE & TECHNOLOGY
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| ISSN | 0013-936X |
| Volume | 56Issue:9Pages:5706-5713 |
| Abstract | Silver nanoparticles (AgNPs) have been widely used in biomedical and consumer products. It remains challenging to distinguish the toxicity of AgNPs derived from the particle form or the released silver ions (Ag+ ). In this study, the toxic effects of two citrate-coated AgNPs (20 and 100 nm) and Ag+ were investigated in hepatoblastoma cells (HepG2 cells). The suppression tests showed that AgNPs and Ag+ induced cell apoptosis via different pathways, which led us to speculate on the AgNPinduced mitochondrial damage. Then, the mitochondrial damages induced by AgNPs and Ag+ were compared under the same intracellular Ag+ concentration, showing that the mitochondrial damage might be mainly attributed to Ag nanoparticles but not to Ag+ . The interaction between AgNPs and mitochondria was analyzed using a scattered light imaging method combined with light intensity profiles and transmission electron microscopy. The colocalization of AgNPs and mitochondria was observed in both NP20- and NP100-treated HepG2 cells, indicating a potential direct interaction between AgNPs and mitochondria. These results together showed that AgNPs induced apoptosis in HepG2 cells through the particle-specific effects on mitochondria. |
| Department | 环境纳米技术与健康效应重点实验室;环境化学与生态毒理学国家重点实验室 ; 环境纳米技术与健康效应重点实验室;环境化学与生态毒理学国家重点实验室 |
| Keyword | CELLULAR UPTAKE CYTOTOXICITY TOXICITY RELEASE QUANTIFICATION MECHANISM DAMAGE |
| Document Type | 期刊论文 |
| Identifier | https://ir.rcees.ac.cn/handle/311016/47665 |
| Collection | 环境纳米技术与健康效应重点实验室 |
| Corresponding Author | Song, Maoyong |
| Affiliation | 1.Chinese Acad Sci, Res Ctr Ecoenvironm Sci, Key Lab Environm Nanotechnol & Hlth Effects, Beijing 100085, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Chinese Acad Sci, Res Ctr Ecoenvironm Sci, State Key Lab Environm Chem & Ecotoxicol, Beijing 100085, Peoples R China |
| Recommended Citation GB/T 7714 | Wang, Fengbang,Chen, Zihan,Wang, Yuanyuan,et al. Silver Nanoparticles Induce Apoptosis in HepG2 Cells through Particle-Specific Effects on Mitochondria[J]. ENVIRONMENTAL SCIENCE & TECHNOLOGY,2022,56(9):5706-5713. |
| APA | Wang, Fengbang.,Chen, Zihan.,Wang, Yuanyuan.,Ma, Chunyan.,Bi, Lei.,...&Jiang, Guibin.(2022).Silver Nanoparticles Induce Apoptosis in HepG2 Cells through Particle-Specific Effects on Mitochondria.ENVIRONMENTAL SCIENCE & TECHNOLOGY,56(9),5706-5713. |
| MLA | Wang, Fengbang,et al."Silver Nanoparticles Induce Apoptosis in HepG2 Cells through Particle-Specific Effects on Mitochondria".ENVIRONMENTAL SCIENCE & TECHNOLOGY 56.9(2022):5706-5713. |
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| File Name/Size | DocType | Version | Access | License | ||
| Silver Nanoparticles（3528KB） | 期刊论文 | 出版稿 | 开放获取 | CC BY-NC-SA | View Application Full Text | |
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