RCEES OpenIR  > 环境水质学国家重点实验室
Occurrence and transfer characteristics of bla(CTX- M) genes among Escherichia coli in anaerobic digestion systems treating swine waste
Tian, Tiantian; Dai, Shiting; Liu, Dejun; Wang, Yang; Qiao, Wei; Yang, Min; Zhang, Yu
2022-08-15
Source PublicationSCIENCE OF THE TOTAL ENVIRONMENT
ISSN0048-9697
Volume834Issue:0Pages:155321
AbstractLivestock waste is a known reservoir of Escherichia coli (E. coli) carrying clinically important CTX-M-type extendedspectrum beta-lactamase genes (bla(CTX-M)), however, the occurrence and transfer characteristics of blaCTX- M genes during anaerobic digestion (AD) remain unclear. Herein, four full-scale and two parallel lab-scale AD systems treating swine waste under ambient and mesophilic conditions were investigated by both molecular- and culture-based methods to reveal the occurrence and transfer behaviors of bla(CTX- M) genes during AD. Real-time TaqMan polymerase chain reaction revealed 1.3 Chi 10(4)-6.8 Chi 10(5) and 3.0 Chi 10(4)-7.0 Chi 10(5)copies/mL of bla(CTX- M) groups 1 and 9 in all feeding substrates. While AD reduced the absolute abundance of groups 1 and 9 by 0.63-2.24 and 0.08-1.30 log (P < 0.05), 5.0 Chi 10(2) -4.1 Chi 10(3) and 1.1 Chi 10(4) -3.5 Chi 10(4) copies/mL of groups 1 and 9 remained in the anaerobic effluent, respectively. In total, 141 bla(CTX-M-)carrying E. coli isolates resistant to cefotaxime were obtained from the AD reactors. Wholegenome sequencing showed that bla(CTX- M-65) mainly carried by E. coli ST155 was the most frequently detected group 9 subtype in the feeding substrate; whereas bla(CTX- M-14) associated with the dominant clones E. coli ST6802 and ST155 became the major subtype in AD effluent. Furthermore, bla(CTX- M-14) was flanked by Delta IS26 upstream and.IS903B downstream. The Delta IS26-bla(CTX- M-14)-Delta IS903B element was mainly located on the IncHI2 plasmid in E. coli ST48 and ST6802 and also the IncFIB plasmid in ST155 in anaerobic effluent. Conjugation assays showed that the plasmids harboring bla(CTX- M-14) could be successfully transferred at a frequency of 10(-3)-10(-2) cells per recipient cell. This study revealed that bla(CTX- M) genes remained in both the full-scale and lab-scale AD effluents of swine waste. Thus, additional efforts should be implemented to block the discharge and spread of antibiotic resistance genes to the environment.
Department环境水质学国家重点实验室 ; 环境水质学国家重点实验室
KeywordPLASMIDS ENCODING CTX-M-14 SPECTRUM BETA-LACTAMASES CTX-M FOOD ANIMALS RESISTANT PREVALENCE IDENTIFICATION DISSEMINATION COMMUNITY
Document Type期刊论文
Identifierhttps://ir.rcees.ac.cn/handle/311016/47715
Collection环境水质学国家重点实验室
Corresponding AuthorZhang, Yu
Affiliation1.Chinese Acad Sci, Res Ctr Ecoenvironm Sci, State Key Lab Environm Aquat Chem, Beijing 100085, Peoples R China
2.China Agr Univ, Coll Vet Med, Beijing Key Lab Detect Technol Anim Derived Food, Beijing 100193, Peoples R China
3.China Agr Univ, Coll Engn, Beijing 100083, Peoples R China
4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
Recommended Citation
GB/T 7714
Tian, Tiantian,Dai, Shiting,Liu, Dejun,et al. Occurrence and transfer characteristics of bla(CTX- M) genes among Escherichia coli in anaerobic digestion systems treating swine waste[J]. SCIENCE OF THE TOTAL ENVIRONMENT,2022,834(0):155321.
APA Tian, Tiantian.,Dai, Shiting.,Liu, Dejun.,Wang, Yang.,Qiao, Wei.,...&Zhang, Yu.(2022).Occurrence and transfer characteristics of bla(CTX- M) genes among Escherichia coli in anaerobic digestion systems treating swine waste.SCIENCE OF THE TOTAL ENVIRONMENT,834(0),155321.
MLA Tian, Tiantian,et al."Occurrence and transfer characteristics of bla(CTX- M) genes among Escherichia coli in anaerobic digestion systems treating swine waste".SCIENCE OF THE TOTAL ENVIRONMENT 834.0(2022):155321.
Files in This Item: Download All
File Name/Size DocType Version Access License
Occurrence and trans(3310KB)期刊论文出版稿开放获取CC BY-NC-SAView Download
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Tian, Tiantian]'s Articles
[Dai, Shiting]'s Articles
[Liu, Dejun]'s Articles
Baidu academic
Similar articles in Baidu academic
[Tian, Tiantian]'s Articles
[Dai, Shiting]'s Articles
[Liu, Dejun]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Tian, Tiantian]'s Articles
[Dai, Shiting]'s Articles
[Liu, Dejun]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: Occurrence and transfer characteristics of blaCTX-M genes among Escherichia coli in anaerobic digestion systems treating swine waste.pdf
Format: Adobe PDF
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.