| Occurrence and transfer characteristics of bla(CTX- M) genes among Escherichia coli in anaerobic digestion systems treating swine waste | |
Tian, Tiantian ; Dai, Shiting; Liu, Dejun; Wang, Yang; Qiao, Wei; Yang, Min; Zhang, Yu
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| 2022-08-15 | |
| Source Publication | SCIENCE OF THE TOTAL ENVIRONMENT
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| ISSN | 0048-9697 |
| Volume | 834Issue:0Pages:155321 |
| Abstract | Livestock waste is a known reservoir of Escherichia coli (E. coli) carrying clinically important CTX-M-type extendedspectrum beta-lactamase genes (bla(CTX-M)), however, the occurrence and transfer characteristics of blaCTX- M genes during anaerobic digestion (AD) remain unclear. Herein, four full-scale and two parallel lab-scale AD systems treating swine waste under ambient and mesophilic conditions were investigated by both molecular- and culture-based methods to reveal the occurrence and transfer behaviors of bla(CTX- M) genes during AD. Real-time TaqMan polymerase chain reaction revealed 1.3 Chi 10(4)-6.8 Chi 10(5) and 3.0 Chi 10(4)-7.0 Chi 10(5)copies/mL of bla(CTX- M) groups 1 and 9 in all feeding substrates. While AD reduced the absolute abundance of groups 1 and 9 by 0.63-2.24 and 0.08-1.30 log (P < 0.05), 5.0 Chi 10(2) -4.1 Chi 10(3) and 1.1 Chi 10(4) -3.5 Chi 10(4) copies/mL of groups 1 and 9 remained in the anaerobic effluent, respectively. In total, 141 bla(CTX-M-)carrying E. coli isolates resistant to cefotaxime were obtained from the AD reactors. Wholegenome sequencing showed that bla(CTX- M-65) mainly carried by E. coli ST155 was the most frequently detected group 9 subtype in the feeding substrate; whereas bla(CTX- M-14) associated with the dominant clones E. coli ST6802 and ST155 became the major subtype in AD effluent. Furthermore, bla(CTX- M-14) was flanked by Delta IS26 upstream and.IS903B downstream. The Delta IS26-bla(CTX- M-14)-Delta IS903B element was mainly located on the IncHI2 plasmid in E. coli ST48 and ST6802 and also the IncFIB plasmid in ST155 in anaerobic effluent. Conjugation assays showed that the plasmids harboring bla(CTX- M-14) could be successfully transferred at a frequency of 10(-3)-10(-2) cells per recipient cell. This study revealed that bla(CTX- M) genes remained in both the full-scale and lab-scale AD effluents of swine waste. Thus, additional efforts should be implemented to block the discharge and spread of antibiotic resistance genes to the environment. |
| Department | 环境水质学国家重点实验室 ; 环境水质学国家重点实验室 |
| Keyword | PLASMIDS ENCODING CTX-M-14 SPECTRUM BETA-LACTAMASES CTX-M FOOD ANIMALS RESISTANT PREVALENCE IDENTIFICATION DISSEMINATION COMMUNITY |
| Document Type | 期刊论文 |
| Identifier | https://ir.rcees.ac.cn/handle/311016/47715 |
| Collection | 环境水质学国家重点实验室 |
| Corresponding Author | Zhang, Yu |
| Affiliation | 1.Chinese Acad Sci, Res Ctr Ecoenvironm Sci, State Key Lab Environm Aquat Chem, Beijing 100085, Peoples R China 2.China Agr Univ, Coll Vet Med, Beijing Key Lab Detect Technol Anim Derived Food, Beijing 100193, Peoples R China 3.China Agr Univ, Coll Engn, Beijing 100083, Peoples R China 4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| Recommended Citation GB/T 7714 | Tian, Tiantian,Dai, Shiting,Liu, Dejun,et al. Occurrence and transfer characteristics of bla(CTX- M) genes among Escherichia coli in anaerobic digestion systems treating swine waste[J]. SCIENCE OF THE TOTAL ENVIRONMENT,2022,834(0):155321. |
| APA | Tian, Tiantian.,Dai, Shiting.,Liu, Dejun.,Wang, Yang.,Qiao, Wei.,...&Zhang, Yu.(2022).Occurrence and transfer characteristics of bla(CTX- M) genes among Escherichia coli in anaerobic digestion systems treating swine waste.SCIENCE OF THE TOTAL ENVIRONMENT,834(0),155321. |
| MLA | Tian, Tiantian,et al."Occurrence and transfer characteristics of bla(CTX- M) genes among Escherichia coli in anaerobic digestion systems treating swine waste".SCIENCE OF THE TOTAL ENVIRONMENT 834.0(2022):155321. |
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| Occurrence and trans（3310KB） | 期刊论文 | 出版稿 | 开放获取 | CC BY-NC-SA | View Download | |
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