| Molecular functional analyses of larval adhesion in a highly fouling invasive model ascidian | |
Cheng, Jiawei; Li, Shiguo ; Li, Xi; Fu, Ruiying; Huang, Xuena; Zhan, Aibin
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| 2022-09-02 | |
| Source Publication | MARINE BIOLOGY
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| ISSN | 0025-3162 |
| Volume | 169Issue:9Pages:120 |
| Abstract | Successful papillary adhesion is the first essential step for serious ascidian biofouling in marine ecosystems; however, the mechanisms of papillary adhesion have not been fully elucidated. Here, we employed transcriptome sequencing to assess the differentially expressed genes (DEGs) between papillae and remaining body in the larvae of the highly fouling invasive model ascidian Ciona robusta. Enrichment results for DEGs showed that papillae were adhesive structures with the combined functions of substrate recognition, environmental perception, and adhesive protein synthesis and secretion. Two candidate ascidian papilla adhesive proteins (APAPs), APAP-1 and APAP-2, were identified, expressed, and purified in vitro. Surface coating tests showed that APAP-1 was a cohesive protein while APAP-2 was an interfacial protein involved in the adhesion between papillae and material surface. Collectively, the obtained DEGs and adhesive proteins provide candidates to deeply understand molecular mechanisms of underwater adhesion and further develop anti-fouling strategies in marine ecosystems. |
| Department | 中国科学院环境生物技术重点实验室 ; 中国科学院环境生物技术重点实验室 |
| Keyword | CIONA-INTESTINALIS NERVOUS-SYSTEM BALANUS-AMPHITRITE CEMENT PROTEINS SENSORY NEURONS SOLITARY AQUACULTURE SETTLEMENT PAPILLAE BEHAVIOR |
| Document Type | 期刊论文 |
| Identifier | https://ir.rcees.ac.cn/handle/311016/47803 |
| Collection | 中国科学院环境生物技术重点实验室 |
| Affiliation | 1.Chinese Acad Sci, Res Ctr Ecoenvironm Sci, Beijing 100085, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| Recommended Citation GB/T 7714 | Cheng, Jiawei,Li, Shiguo,Li, Xi,et al. Molecular functional analyses of larval adhesion in a highly fouling invasive model ascidian[J]. MARINE BIOLOGY,2022,169(9):120. |
| APA | Cheng, Jiawei,Li, Shiguo,Li, Xi,Fu, Ruiying,Huang, Xuena,&Zhan, Aibin.(2022).Molecular functional analyses of larval adhesion in a highly fouling invasive model ascidian.MARINE BIOLOGY,169(9),120. |
| MLA | Cheng, Jiawei,et al."Molecular functional analyses of larval adhesion in a highly fouling invasive model ascidian".MARINE BIOLOGY 169.9(2022):120. |
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| Molecular functional（4692KB） | 期刊论文 | 出版稿 | 开放获取 | CC BY-NC-SA | View Download | |
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