RCEES OpenIR  > 中国科学院饮用水科学与技术重点实验室
Thyroid Hormone Disruption by Organophosphate Esters Is Mediated by Nuclear/Membrane Thyroid Hormone Receptors: In Vitro, In Vivo, and In Silico Studies
Li, Jian; Xu, Ying; Li, Na; Zuo, Rui; Zhai, Yuanzheng; Chen, Haiyang
2022-04-05
Source PublicationENVIRONMENTAL SCIENCE & TECHNOLOGY
ISSN0013-936X
Volume56Issue:7Pages:4241-4250
AbstractEarlier mechanistic studies of many prohibited flame retardants (FRs) highlighted their thyroid hormone-disrupting activity through nuclear thyroid hormone receptors (nTRs), whereas some alternative FRs such as organophosphate esters (OPEs) exerted weak nTR-disrupting effects. However, an increasing number of studies have revealed that OPEs also exert thyroid hormone-disrupting effects, and the underlying mechanism is unclear. Herein, the thyroid hormone-disrupting effects and mechanisms of 8 typical OPEs were investigated using integrated in vitro, in vivo, and in silico assays. All tested chemicals competitively bound to the membrane thyroid hormone receptor (mTR) [the 20% relative inhibitory concentration (RIC20): (3.5 +/- 0.2) X 10(7) to (4.9 +/- 1.0) X 10(7) nM], and CI-OPEs and alkyl-OPEs had lower RIC20 values. In contrast, only 4 OPEs showed nTR antagonistic activities at higher concentrations [>= (4.8 +/- 0.8) X 10(3) nM]. CI-OPEs and alkyl-OPEs preferentially interacted with mTR. Molecular docking illustrated that OPEs docked into mTRs, consistent with the competitive binding assay. In vivo analyses of zebrafish embryonic development confirmed that tris(1,3-dichloro-2-propyl) phosphate induced inappropriate expression of proteins, and these protein interactions might be associated with mTR according to the quantitative proteomic analysis. Based on the results, mTR might play a critical role in mediating the thyroid hormone-disrupting effects of OPEs.
Department中国科学院饮用水科学与技术重点实验室 ; 中国科学院饮用水科学与技术重点实验室
KeywordFLAME RETARDANTS INTEGRIN ALPHA(V)BETA(3) TRIPHENYL PHOSPHATE EXPOSURE ALPHA-V-BETA-3 EMBRYOS/LARVAE ACTIVATION TOXICITY ANIMALS TDCPP
Document Type期刊论文
Identifierhttps://ir.rcees.ac.cn/handle/311016/47910
Collection中国科学院饮用水科学与技术重点实验室
Corresponding AuthorLi, Jian
Affiliation1.Beijing Normal Univ, Coll Water Sci, Engn Res Ctr Groundwater Pollut Control & Remedia, Minist Educ, Beijing 100875, Peoples R China
2.Chinese Acad Sci, Res Ctr Ecoenvironm Sci, Key Lab Drinking Water Sci & Technol, Beijing 100085, Peoples R China
Recommended Citation
GB/T 7714
Li, Jian,Xu, Ying,Li, Na,et al. Thyroid Hormone Disruption by Organophosphate Esters Is Mediated by Nuclear/Membrane Thyroid Hormone Receptors: In Vitro, In Vivo, and In Silico Studies[J]. ENVIRONMENTAL SCIENCE & TECHNOLOGY,2022,56(7):4241-4250.
APA Li, Jian,Xu, Ying,Li, Na,Zuo, Rui,Zhai, Yuanzheng,&Chen, Haiyang.(2022).Thyroid Hormone Disruption by Organophosphate Esters Is Mediated by Nuclear/Membrane Thyroid Hormone Receptors: In Vitro, In Vivo, and In Silico Studies.ENVIRONMENTAL SCIENCE & TECHNOLOGY,56(7),4241-4250.
MLA Li, Jian,et al."Thyroid Hormone Disruption by Organophosphate Esters Is Mediated by Nuclear/Membrane Thyroid Hormone Receptors: In Vitro, In Vivo, and In Silico Studies".ENVIRONMENTAL SCIENCE & TECHNOLOGY 56.7(2022):4241-4250.
Files in This Item: Download All
File Name/Size DocType Version Access License
Thyroid Hormone Disr(2810KB)期刊论文出版稿开放获取CC BY-NC-SAView Download
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Li, Jian]'s Articles
[Xu, Ying]'s Articles
[Li, Na]'s Articles
Baidu academic
Similar articles in Baidu academic
[Li, Jian]'s Articles
[Xu, Ying]'s Articles
[Li, Na]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Li, Jian]'s Articles
[Xu, Ying]'s Articles
[Li, Na]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: Thyroid Hormone Disruption by Organophosphate Esters Is Mediated by Nuclear_Membrane Thyroid Hormone Receptors_ In Vitro, In Vivo, and In Silico Studies.pdf
Format: Adobe PDF
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.