| Low concentrations of benzophenone-type UV-filters impair testis development in the amphibian Xenopus laevis | |
Li, Hong-Mei; Zhang, Ying-Chi; Li, Yuan -Yuan; Zhu, Qing-Qing ; Li, Jie; Xu, Hai-Ming; Xiong, Yi-Ming; Qin, Zhan-Fen
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| 2023 | |
| Source Publication | AQUATIC TOXICOLOGY
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| ISSN | 0166-445X |
| Volume | 254Pages:106371-1-9 |
| Abstract | Benzophenone-type UV filters (BPs) are ubiquitous contaminants in aquatic environments, possibly posing ecological risks to aquatic populations. So far, little is known about the potential adverse effects of BPs on amphibians. Given their potential estrogenic property, we investigated the detrimental effects of the commonly used BPs, BP-3, BP-2, and BP-1, on testis development in amphibians using Xenopus laevis as a model species. Following exposure to 10, 100, 1000 nM BP-3, BP-2, or BP-1 from stages 45/46 to 52, tadpoles presented morphological abnormal testes, characterized by reduced gonomere size and testis area, coupled with suppressed cell proliferation. Meanwhile, the downregulation of testis-biased gene expression and the upregulation of ovary -biased gene expression were observed in BPs-treated testes. Moreover, the estrogen receptor (ER) antagonist ICI 182780 significantly antagonized ovary-biased gene upregulation caused by BPs, suggesting that the effects of BPs on testis differentiation could be mediated by ER, at least partially. Of note, the effects of BPs were not concentration-dependent, but the lowest concentration generally exerted significant effects. Altogether, these observations indicate that the three BPs inhibited testis differentiation and exerted feminizing effects. Impor-tantly, when BP-2 exposure was extended to two months post-metamorphosis, testes of froglets were generally less-developed, with relatively fewer spermatocytes, more spermatogonia, and poorly formed seminiferous tu-bules. Considering the fact that the lowest concentration (10 nM) of BPs in this study are detectable in aquatic environments, we conclude that BP-3, BP-2, and BP-1, even at environmentally relevant concentrations, can retard testis differentiation at pre-metamorphic stages and cause testis dysgenesis after metamorphosis in the amphibian X. laevis. Our findings suggest that ubiquitous BPs in aquatic environments could pose a potential risk to amphibians. |
| Department | 环境化学与生态毒理学国家重点实验室 |
| Keyword | Benzophenones Xenopus laevis Testis development Feminizing Amphibians |
| Document Type | 期刊论文 |
| Identifier | https://ir.rcees.ac.cn/handle/311016/48500 |
| Collection | 环境化学与生态毒理学国家重点实验室 |
| Affiliation | 1.Chinese Academy of Sciences 2.Research Center for Eco-Environmental Sciences (RCEES) 3.University of Chinese Academy of Sciences, CAS 4.Ningxia Medical University |
| Recommended Citation GB/T 7714 | Li, Hong-Mei,Zhang, Ying-Chi,Li, Yuan -Yuan,et al. Low concentrations of benzophenone-type UV-filters impair testis development in the amphibian Xenopus laevis[J]. AQUATIC TOXICOLOGY,2023,254:106371-1-9. |
| APA | Li, Hong-Mei.,Zhang, Ying-Chi.,Li, Yuan -Yuan.,Zhu, Qing-Qing.,Li, Jie.,...&Qin, Zhan-Fen.(2023).Low concentrations of benzophenone-type UV-filters impair testis development in the amphibian Xenopus laevis.AQUATIC TOXICOLOGY,254,106371-1-9. |
| MLA | Li, Hong-Mei,et al."Low concentrations of benzophenone-type UV-filters impair testis development in the amphibian Xenopus laevis".AQUATIC TOXICOLOGY 254(2023):106371-1-9. |
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