| Toward efficient bioremediation of methylmercury in sediment using merB overexpressed Escherichia coli | |
Yang, Qingqing ; Guo, Yingying; Xiang, Yuping; Chen, Lufeng; Liu, Guangliang; Liu, Yanwei; Shi, Jianbo; Hu, Ligang; Liang, Yong; Yin, Yongguang; Cai, Yong; Jiang, Guibin
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| 2023 | |
| Source Publication | WATER RESEARCH
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| ISSN | 0043-1354 |
| Volume | 229Pages:119502-1-9 |
| Abstract | Sediment is the primary hotspot for microbial production of toxic and bio-accumulative methylmercury (MeHg). Common remediation strategies such as sediment dredging and capping can be too expensive and cannot degrade MeHg efficiently. Here, we constructed an Escherichia coli strain overexpressing merB gene (DH5 alpha J23106) and assessed the effectiveness of this recombinant strain in degradation of MeHg in culture medium and sediment. DH5 alpha J23106 can efficiently degrade MeHg (with initial concentration from 0.01 to 50 ng/mL) to more than 81.6% in a culture medium under anoxic and oxic conditions. Enriched isotope addition (199HgCl2) revealed that this recombinant strain can degrade 78.6% of newly produced Me199Hg in actual sediment, however the biodegradation decreased to 36.3% for intrinsic MeHg. Degradation of spiked MeHg after aging in anoxic and oxic sediments further demonstrated DH5 alpha J23106 can efficiently degrade newly produced MeHg and the degradation decreased with aging significantly, especially for oxic sediment. Eight sediments were further assessed for the biodegradation of aged MeHg by DH5 alpha J23106 under oxic conditions, with degradation ratios ranging from 9.0% to 66.9%. When combined with (NH4)2S2O3leaching, the degradation of MeHg increased by 15.8-38.8% in on-site and off-site modes through enhanced MeHg bioavailability in some of these sediments. Thus, this recombinant strain DH5 alpha J23106 can degrade MeHg efficiently and have the potential for remediating bioavailable MeHg in contaminated sediments. |
| Department | 环境化学与生态毒理学国家重点实验室 |
| Keyword | Methylmercury Microbial remediation MeHg Sediment Ammonium thiosulfate Bioavailability |
| Document Type | 期刊论文 |
| Identifier | https://ir.rcees.ac.cn/handle/311016/48520 |
| Collection | 环境化学与生态毒理学国家重点实验室 |
| Affiliation | 1.Chinese Academy of Sciences 2.Research Center for Eco-Environmental Sciences (RCEES) 3.University of Chinese Academy of Sciences, CAS 4.Jianghan University 5.State University System of Florida 6.Florida International University |
| Recommended Citation GB/T 7714 | Yang, Qingqing,Guo, Yingying,Xiang, Yuping,et al. Toward efficient bioremediation of methylmercury in sediment using merB overexpressed Escherichia coli[J]. WATER RESEARCH,2023,229:119502-1-9. |
| APA | Yang, Qingqing.,Guo, Yingying.,Xiang, Yuping.,Chen, Lufeng.,Liu, Guangliang.,...&Jiang, Guibin.(2023).Toward efficient bioremediation of methylmercury in sediment using merB overexpressed Escherichia coli.WATER RESEARCH,229,119502-1-9. |
| MLA | Yang, Qingqing,et al."Toward efficient bioremediation of methylmercury in sediment using merB overexpressed Escherichia coli".WATER RESEARCH 229(2023):119502-1-9. |
| Files in This Item: | ||||||
| File Name/Size | DocType | Version | Access | License | ||
| Toward efficient bio（1329KB） | 期刊论文 | 出版稿 | 开放获取 | CC BY-NC-SA | View Application Full Text | |
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