RCEES OpenIR  > 城市与区域生态国家重点实验室
GeoChip 4: a functional gene-array-based high-throughput environmental technology for microbial community analysis
Tu, Qichao; Yu, Hao; He, Zhili; Deng, Ye; Wu, Liyou; Van Nostrand, Joy D.; Zhou, Aifen; Voordeckers, James; Lee, Yong-Jin; Qin, Yujia; Hemme, Christopher L.; Shi, Zhou; Xue, Kai; Yuan, Tong; Wang, Aijie; Zhou, Jizhong
2014
Source PublicationMOLECULAR ECOLOGY RESOURCES
ISSN1755-098X
Volume14Issue:5Pages:914-928
AbstractMicro-organisms play critical roles in many important biogeochemical processes in the Earth's biosphere. However, understanding and characterizing the functional capacity of microbial communities are still difficult due to the extremely diverse and often uncultivable nature of most micro-organisms. In this study, we developed a new functional gene array, GeoChip 4, for analysing the functional diversity, composition, structure, metabolic potential/activity and dynamics of microbial communities. GeoChip 4 contained approximately 82 000 probes covering 141 995 coding sequences from 410 functional gene families related to microbial carbon (C), nitrogen (N), sulphur (S), and phosphorus (P) cycling, energy metabolism, antibiotic resistance, metal resistance/reduction, organic remediation, stress responses, bacteriophage and virulence. A total of 173 archaeal, 4138 bacterial, 404 eukaryotic and 252 viral strains were targeted, providing the ability to analyse targeted functional gene families of micro-organisms included in all four domains. Experimental assessment using different amounts of DNA suggested that as little as 500 ng environmental DNA was required for good hybridization, and the signal intensities detected were well correlated with the DNA amount used. GeoChip 4 was then applied to study the effect of long-term warming on soil microbial communities at a Central Oklahoma site, with results indicating that microbial communities respond to long-term warming by enriching carbon degradation, nutrient cycling (nitrogen and phosphorous) and stress response gene families. To the best of our knowledge, GeoChip 4 is the most comprehensive functional gene array for microbial community analysis.
Department城市与区域生态国家重点实验室
KeywordEnvironmental Technology Functional Gene Array Geochip 4 Microbial Community Analysis
Citation statistics
Document Type期刊论文
Identifierhttps://ir.rcees.ac.cn/handle/311016/9163
Collection城市与区域生态国家重点实验室
Recommended Citation
GB/T 7714
Tu, Qichao,Yu, Hao,He, Zhili,et al. GeoChip 4: a functional gene-array-based high-throughput environmental technology for microbial community analysis[J]. MOLECULAR ECOLOGY RESOURCES,2014,14(5):914-928.
APA Tu, Qichao.,Yu, Hao.,He, Zhili.,Deng, Ye.,Wu, Liyou.,...&Zhou, Jizhong.(2014).GeoChip 4: a functional gene-array-based high-throughput environmental technology for microbial community analysis.MOLECULAR ECOLOGY RESOURCES,14(5),914-928.
MLA Tu, Qichao,et al."GeoChip 4: a functional gene-array-based high-throughput environmental technology for microbial community analysis".MOLECULAR ECOLOGY RESOURCES 14.5(2014):914-928.
Files in This Item: Download All
File Name/Size DocType Version Access License
GeoChip 4 a function(403KB) 开放获取--View Application Full Text
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Tu, Qichao]'s Articles
[Yu, Hao]'s Articles
[He, Zhili]'s Articles
Baidu academic
Similar articles in Baidu academic
[Tu, Qichao]'s Articles
[Yu, Hao]'s Articles
[He, Zhili]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Tu, Qichao]'s Articles
[Yu, Hao]'s Articles
[He, Zhili]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: GeoChip 4 a functional gene-array-based high-throughput environmental technology for microbial community analysis.pdf
Format: Adobe PDF
This file does not support browsing at this time
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.