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Functional metagenomic characterization of antibiotic resistance genes in agricultural soils from China
Su, Jian Qiang; Wei, Bei; Xu, Chun Yan; Qiao, Min; Zhu, Yong Guan
2014
Source PublicationENVIRONMENT INTERNATIONAL
ISSN0160-4120
Volume65Issue:1Pages:9-15
AbstractSoil has been regarded as a rich source of antibiotic resistance genes (ARGs) due to the complex microbial community and diverse antibiotic-producing microbes in soil, however, little is known about the ARGs in unculturable bacteria. To investigate the diversity and distribution of ARGs in soil and assess the impact of agricultural practice on the ARGs, we screened soil metagenomic library constructed using DNA from four different agricultural soil for ARGs. We identified 45 clones conferring resistance to minocycline, tetracycline, streptomycin, gentamicin, kanamycin, amikacin, chloramphenicol and rifampicin. The similarity of identified ARGs with the closest protein in GenBank ranged from 26% to 92%, with more than 60% of identified ARGs had low similarity less than 60% at amino acid level. The identified ARGs include aminoglycoside acetyltransferase, aminoglycoside 6-adenyltransferase, ADP-ribosyl transferase, ribosome protection protein, transporters and other antibiotic resistant determinants. The identified ARGs from the soil with manure application account for approximately 70% of the total ARGs in this study, implying that manure amendment may increase the diversity of antibiotic resistance genes in soil bacteria. These results suggest that antibiotic resistance in soil remains unexplored and functional metagenomic approach is powerful in discovering novel ARGs and resistant mechanisms. (C) 2013 Elsevier Ltd. All rights reserved.
Department土壤环境科学实验室
KeywordAntibiotic Resistance Functional Metagenomics Agricultural Soils
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Document Type期刊论文
Identifierhttps://ir.rcees.ac.cn/handle/311016/9629
Collection土壤环境科学实验室
Recommended Citation
GB/T 7714
Su, Jian Qiang,Wei, Bei,Xu, Chun Yan,et al. Functional metagenomic characterization of antibiotic resistance genes in agricultural soils from China[J]. ENVIRONMENT INTERNATIONAL,2014,65(1):9-15.
APA Su, Jian Qiang,Wei, Bei,Xu, Chun Yan,Qiao, Min,&Zhu, Yong Guan.(2014).Functional metagenomic characterization of antibiotic resistance genes in agricultural soils from China.ENVIRONMENT INTERNATIONAL,65(1),9-15.
MLA Su, Jian Qiang,et al."Functional metagenomic characterization of antibiotic resistance genes in agricultural soils from China".ENVIRONMENT INTERNATIONAL 65.1(2014):9-15.
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